Rheostatic Control of Cas9-Mediated DNA Double Strand Break (DSB) Generation and Genome Editing
نویسندگان
چکیده
منابع مشابه
Rheostatic Control of Cas9-Mediated DNA Double Strand Break (DSB) Generation and Genome Editing.
We recently reported two novel tools for precisely controlling and quantifying Cas9 activity: a chemically inducible Cas9 variant (ciCas9) that can be rapidly activated by small molecules and a ddPCR assay for time-resolved measurement of DNA double strand breaks (DSB-ddPCR). Here, we further demonstrate the potential of ciCas9 to function as a tunable rheostat for Cas9 function. We show that a...
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ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2017
ISSN: 1554-8929,1554-8937
DOI: 10.1021/acschembio.7b00652